CRHR2 ELISA Kit for Human(Homo sapiens) | EIAab.comCRHR2 ELISA Kit for Human(Homo sapiens)

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Index > Protein center > CRHR2(Gene name) > Human

CRHR2 (Gene name),

Corticotropin-releasing factor receptor 2 (Protein name ), CRFR2_HUMAN from NCBI database.

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General Annotation

Antigen Annotation

Predicted Eptitope

Vaild Sequence

Related Databases

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Gene name:

CRHR2(CRF2R;CRH2R);

Protein name:

Corticotropin-releasing factor receptor 2(CRF-R-2;CRF-R2;CRFR-2);

Alternative:

Corticotropin-releasing hormone receptor 2(CRH-R-2;CRH-R2);

Organism:

Human (Homo sapiens).

General Annotation

Sub Unit:

Interacts (via N-terminal extracellular domain) with CRF, UCN, UCN2 and UCN3. Has highest affinity for UCN, and considerably lower affinity for CRF, UNC2 and UCN3.

Function:

This is a receptor for corticotropin releasing factor. Shows high-affinity CRF binding. Also binds to urocortin I, II and III. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase.

Subcellular Location:

Cell membrane Multi-pass membrane protein

Protein Attributes:

Sequence length:

411

Sequence:

50:

100:

150:

200:

250:

300:

350:

400:

411:

SFHSIKQTAA | V

3D Structure:

N/A

Predicted Eptitope:

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Vaild Sequence:

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Related Databases

String:

Q13324

STRING:

9606.ENSP00000222836

KEGG:

hsa:1395

UniGene:

Hs.546246

Pfam:

PF00002

SMR:

Q13324

MIM:

602034

Uniprot:

Q13324

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ELISA Kit for Human CRF-R-2

Cat.:

E0241h

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96T

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ELISA Kit for Human CRF-R-2

Cat.:

E0241r

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96T

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ELISA Kit for Human CRF-R-2

Cat.:

E0241m

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96T

CLIA Kit for Human CRF-R-2

Cat.:

U0241h

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96T

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CLIA Kit for Human CRF-R-2

Cat.:

U0241r

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96T

CLIA Kit for Human CRF-R-2

Cat.:

U0241m

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96T

Polyclonal Antibody for Human CRF-R-2

Cat.:

P0241Rb-m

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Packing:

40ug/0.2ml

Polyclonal Antibody for Human CRF-R-2

Cat.:

P0241Rb-r

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40ug/0.2ml

Polyclonal Antibody for Human CRF-R-2

Cat.:

P0241Rb-h

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40ug/0.2ml

Monoclonal Antibody for Human CRF-R-2

Protein for Human CRF-R-2

R&D Technical Data

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤3.7%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤6.5%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Recovery

Recovery was determined by spiking various levels of CRFR2 into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	99	93-103
Plasma	98	95-100

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CRFR2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	95-100%	96-102%	95-99%	97-104%
EDTA plasma(n=5)	93-97%	95-100%	92-100%	96-102%
heparin plasma(n=5)	95-104%	93-104%	102-108%	91-97%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

References

1.

"Cloning and characterization of the human corticotropin-releasing factor-2 receptor complementary deoxyribonucleic acid."
Liaw C.W. , Lovenberg T.W. , Barry G. , Oltersdorf T. , Grigoriadis D.E. , de Souza E.B.
Endocrinology137:72-77(1996) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM CRF2-ALPHA)
2.

"Molecular cloning of the human CRH2B receptor isoform: divergence from the rodent isoform in sequence and expression pattern."
Kostich W.A. , Chen A. , Sperle K. , Horlick R.A. , Patterson J. , Hyde T.M. , Largent B.L.
Submitted (1997-06) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM CRF2-BETA)
tissue: Amygdala.
3.

"Molecular identification and analysis of a novel human corticotropin-releasing factor (CRF) receptor: the CRF2gamma receptor."
Kostich W.A. , Chen A. , Sperle K. , Largent B.L.
Mol. Endocrinol.12:1077-1085(1998) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM CRF2-GAMMA)
tissue: Amygdala.
4.

"Genome-wide discovery and analysis of human seven transmembrane helix receptor genes."
Suwa M. , Sato T. , Okouchi I. , Arita M. , Futami K. , Matsumoto S. , Tsutsumi S. , Aburatani H. , Asai K. , Akiyama Y.
Submitted (2001-07) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM CRF2-ALPHA)
5.

"cDNA clones of human proteins involved in signal transduction sequenced by the Guthrie cDNA resource center (www.cdna.org)."
King M.M. , Aronstam R.S. , Sharma S.V.
Submitted (2003-10) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM CRF2-ALPHA)
tissue: Brain.
6.

"Identification and characterization of novel CRF receptor type 2 isoforms in human."
Wu S.V. , Yuan P.-Q. , Lai J. , Tache Y.
Submitted (2007-07) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS D; E; F AND DESQ);ALTERNATIVE SPLICING
7.

"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T. , Suzuki Y. , Nishikawa T. , Otsuki T. , Sugiyama T. , Irie R. , Wakamatsu A. , Hayashi K. , Sato H. , Nagai K. , Kimura K. , Makita H. , Sekine M. , Obayashi M. , Nishi T. , Shibahara T. , Tanaka T. , Ishii S. , more...
Nat. Genet.36:40-45(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM CRF2-ALPHA)
tissue: Amygdala.
8.

"The DNA sequence of human chromosome 7."
Hillier L.W. , Fulton R.S. , Fulton L.A. , Graves T.A. , Pepin K.H. , Wagner-McPherson C. , Layman D. , Maas J. , Jaeger S. , Walker R. , Wylie K. , Sekhon M. , Becker M.C. , O'Laughlin M.D. , Schaller M.E. , Fewell G.A. , Delehaunty K.D. , Miner T.L. , more...
Nature424:157-164(2003) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]
9.

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM CRF2-ALPHA)
10.

"A new functional isoform of the human CRF2 receptor for corticotropin-releasing factor."
Valdenaire O. , Giller T. , Breu V. , Gottowik J. , Kilpatrick G.
Biochim. Biophys. Acta1352:129-132(1997) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-88 (ISOFORM CRF2-BETA)
tissue: Skeletal muscle.
11.

"The Pseudo signal peptide of the corticotropin-releasing factor receptor type 2A prevents receptor oligomerization."
Teichmann A. , Rutz C. , Kreuchwig A. , Krause G. , Wiesner B. , Schulein R.
J. Biol. Chem.287:27265-27274(2012) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: SUBUNIT;SUBCELLULAR LOCATION;NON-CLEAVABLE SIGNAL SEQUENCE
12.

"Structural basis for hormone recognition by the Human CRFR2{alpha} G protein-coupled receptor."
Pal K. , Swaminathan K. , Xu H.E. , Pioszak A.A.
J. Biol. Chem.285:40351-40361(2010) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS) OF 3-104 IN COMPLEXES WITH UCN; UCN2 AND UCN3;INTERACTION WITH CRF; UCN; UCN2 AND UCN3;DISULFIDE BONDS

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